Fig. 6.

Case Q294, sacrificed at HH35, processed for Tbr1 ISH and QCPN IMR, and sectioned sagittally. a Drawing of the graft extent relative to the prosencephalic fate map. b Schema of the Q294 chimeric brain, illustrating the plane of the sections shown in (c–l). c Detail of the PThE and the juxtapeduncular and eminentio-septal streams at the commissural septum level. d-e Higher magnification of the areas framed in a black and a red square in (c); observe quail cells (red dots) in the Tbr1-positive area. f Detail of a parasagittal section alevel with the peduncle entering the subpallium. g–j Higher magnifications of colored framed areas in (f). k Section of the telencephalon at the level of the extended amygdala (EA). l Higher magnification of the red framed area in (k), where red arrowheads point to peripeduncular migrated quail cells. Blue arrowheads indicate the ventricular borders of the graft. Red arrowheads point to the peripeduncular stream. Black arrowheads point to the juxtapeduncular stream. Green arrowheads point to the eminentio-septal stream. Scale bars in a, 250 μm, b, 2.5 mm, c, f and k, 150 μm, d–e, g–j and l, 50 μm