Fig. 7.

Case M25, sacrificed at HH38, processed for Tbr1 ISH and QCPN IMR, and sectioned in a plane orthogonal to the peduncle. a Drawing of graft extent relative to the prosencephalic fate map. b Schema of the M25 chimeric brain, illustrating the plane of the sections shown in (c–q). c–g Ventrodorsal series through the diencephalic-telencephalic transition. h–i Higher magnifications of red framed areas in (d). j–k Higher magnification of red framed areas in (e). l–m Higher magnification of red framed areas in (f). n Higher magnification of red framed area in (g). o–q Details of three consecutive sections situated immediately dorsal to (n). Blue arrowheads indicate the ventricular borders of the graft. Red arrowheads point to the peripeduncular stream. Black arrowheads point to the juxtapeduncular stream. Green arrowheads point to the eminentio-septal stream. Scale bars in a, 250 μm, b, 5 mm, c-g, 150 μm, h-m, 50 μm, n-q, 150 μm