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. 2021 Mar 21;22:199. doi: 10.1186/s12864-021-07537-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The correlation of ovary samples at different stages of P. clarkii based on the RNA-seq analysis. a: The heatmap of the Pearson coefficients among all the ovary samples. The more grey the color of the block, the lower correlation between two samples; the more yellow the color, the higher correlation between two samples. b: The Principal Component Analysis (PCA) of all the twelve ovary samples. c: Hierarchical clustering based on the distances among the twelve samples. The length of the branch represents the distance between the two samples, the more similar the samples, the closer they get. d: The sample distance boxplot of the four stages. e: The analysis of similarity (Anosim) boxplot among different groups. Note: The alphabet of a, b, c and d in the graph represent the stage I, stage II, stage III and stage IV, respectively