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. Author manuscript; available in PMC: 2022 Apr 2.
Published in final edited form as: Biochem Biophys Res Commun. 2021 Feb 13;547:29–35. doi: 10.1016/j.bbrc.2021.01.103

Fig. 1. Regulation of TRB3 expression in mouse brown adipose tissue.

Fig. 1.

(a) Male C57BL/6 mice (8 weeks old) were fed a high fat diet for 6 weeks. Brown adipose tissue was dissected to determine mRNA expression of TRB3 and other genes involved in brown adipose tissue differentiation (n=5–6). (b) Male ob/ob mice and their controls (12 weeks old) were euthanized and brown adipose tissue was collected to determine mRNA expression of TRB3 and genes involved in brown adipose tissue differentiation (n=4). (c and d) Brown preadipocytes were differentiated 6 days and incubated with 0, 0.25, or 0.75 mM of palmitate with 2% BSA for 16h to determine TRB3 (c) and UCP1 (d) expression (n=4). * indicates p<0.05, ** indicates p<0.01, and *** indicates p<0.001 vs. control.