Figure 1.

Subcellular localization of IBV E and M proteins in transfected Cos-7 cells. The T7-tagged E and M expressed in Cos-7 cells were detected using monoclonal anti-T7 (A) and polyclonal anti-M antibodies (D andG), respectively. The proteins were then labeled with the FITC-conjugated secondary antibodies. Immunofluorescent staining of E (A) and M (D and G) gives a reticular staining pattern and a perinuclear, Golgi-like staining pattern, respectively. B and E refer to cells stained with R6, a dye for the ER, and H refers to a cell stained with BODIPY TR-ceramide (TR-C), a vital dye for the Golgi apparatus. The green images represent the FITC-derived green fluorescence, and red images represent the rhodamine and Texas Red-derived red fluorescence. Colocalization of viral proteins with the organelle markers is represented by theyellow region within each cell in the merged images (C, F, and I). The fluorescence was viewed using a confocal scanning Zeiss microscope.