Figure 2.

Subcellular localization and translocation of E protein. Cos-7 cells expressing E were incubated in the presence of 100 μg/ml cycloheximide for 30 min at 3.5 h post-transfection. The cells were fixed at 4, 7, 10, and 16 h and subjected to indirect immunofluorescent staining with anti-T7 monoclonal antibody followed by incubating with FITC-conjugated anti-mouse antiserum. Immunostaining of E is presented in A,D, G, and J. B andE show the ER staining with R6; H shows the BODIPY TR-ceramide (TR-C) staining pattern; and Kshows the LysoTracker^TM Red DND-99 (LT) staining pattern. The green images represent the FITC-derived green fluorescence, and red images represent the Rhodamine and Texas Red-derived red fluorescence. Colocalization of viral proteins with the organelle markers is represented by theyellow region within each cell in the merged images (C, F, I, and L).