Figure 7.

The effects of coexpression of E with M on the subcellular localization of M. M was coexpressed with E in Cos-7 cells, incubated in the presence of 100 μg/ml of cycloheximide for 30 min, and subjected to indirect immunofluorescent staining. The cells were fixed at 4 (A–C), 7 (D–F), 10 (G–I), and 16 (J–L) h, respectively, and the subcellular localization of proteins were examined by dual labeling with a mixture of anti-T7 (mouse) and anti-M (rabbit) antisera, followed by incubating with a mixture of FITC-conjugated anti-mouse and tetramethyl rhodamine isocyanate-conjugated anti-rabbit antisera. The staining patterns of E are indicated by the green images (A, D, G, and J), and the staining patterns of M are indicated by the red images (B, E, H, K, N, and Q). M and P refer to cells expressing EΔ2 and EΔ7, respectively. Merged images (yellow) represent colocalization of the two proteins (C, F, I, L, O, and R).