Figure 4.
Tamoxifen-induced deletion of neuronal Rac1 reduced axonal regeneration with no effect on tissue loss after ischemic stroke in mice. Density assessment of (A) neurofilament-L (NFL, green, Alexa Fluor 647, N = 6/each group) and (B) myelin basic protein (MBP, red, Alexa Fluor 594, N = 7/each group) in the ipsilateral (ipsi) and contralateral (contra) hemisphere 28 days after unilateral stroke. Blue indicated cell nucleus staining using DAPI. (C) Quantification of brain tissue loss 28 days after stroke was assayed by CV staining (N = 5/each group). N = number of animals. *p < 0.05, **p < 0.01, ***p < 0.001. Two-way ANOVA with subsequent Bonferonni test was applied for immune-staining. Mann-Whitney test was used for CV staining.