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. 2020 Aug 7;11(3):823–834. doi: 10.1016/j.apsb.2020.07.021

Figure 4.

Figure 4

The viability of MDA-MB-231 cells detected by MTT assay after incubating with (A) drug-free carriers (GO and GO@MH). (B) GO/Ce6 and GO/Ce6@MH with 1 min laser (100 mW) irradiation for 48 h, ∗∗P < 0.01. (C) The fluorescence images of ROS by DCFDA staining in MDA-MB-231 cells treated with various formulations upon laser irradiation. The concentration of HA was 10 mg/mL. (D) The fluorescence quantitative analysis of ROS level inside cells. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. The cell viability after incubating with (E) GO/CisPt and GO/CisPt@MH (F) GO/CisPt/Ce6 + Laser, GO/CisPt/Ce6@MH and GO/CisPt/Ce6@MH + Laser. ∗∗P < 0.01, ∗∗∗P < 0.001. (G) The fluorescence images of MDA-MB-231 cells underwent different treatments co-stained by calcein-AM/PI. Data are expressed as mean ± SD (n = 4).