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. 2020 Nov 1;11(3):680–693. doi: 10.1016/j.apsb.2020.10.017

Figure 8.

Figure 8

Isor alleviated Dox-induced cardiomyocytes apoptosis and increased YAP1 expression in vivo. The heart tissue of mice was stained with TUNEL and observed by EVOS FL Auto. Representative graphs are shown and scale bar is 100 μm (A). The change of cleaved caspase 3 levels in the heart tissue of mice was measured by Western blot (B). The protein level of YAP1 in the heart of mice was assayed by IHC and scale bar is 100 μm (C). The protein and mRNA levels of Yap1 in the heart of mice were measured by Western blot (D) and qRT-PCR (E). The mRNA expressions of YAP1′ target genes (Ctgf and Areg) were assayed by qRT-PCR (F) and (G). Data were expressed as mean ± SEM, n = 6; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. control group; #P < 0.05, ##P < 0.01 vs. Dox group. YAP1, Yes-associated protein 1; Isor, isorhapontigenin; Dox, doxorubicin; IHC, immunohistochemistry; qRT-PCR, quantitative real-time polymerase chain reaction; CTGF, connective tissue growth factor; AREG, amphiregulin.