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. 2021 Mar 8;9:647387. doi: 10.3389/fcell.2021.647387

FIGURE 3.

FIGURE 3

LMO7 defect-caused suppression of cell proliferation and colony formation in Panc02-H7 cells. (A) Schematic diagram for assay of LMO7-mediated cell proliferation and colony formation. (B) MTT detected the suppression of Panc02-H7 cell proliferation post knockdown of LMO7 with siRNAs. (C) Crystal violet staining detected the reduced cell colonies in Panc02-H7 cells post knockdown of LMO7 with siRNAs. (E–G) The reduced cell proliferation and colony formation in stable LMO7-shRNA-Panc02-H7 were detected with the same methods described in (B) to (D). (H–J) The reduced cell proliferation and colony formation in stable LMO7-CRISPR-Panc02-H7 cells were detected with the same methods described in (B) to (D). n = 3, error bars represent mean ± SD. **p < 0.01; ***p < 0.001.