Table 4.
Experimental conditions of live imaging of microglia in vitro.
| Animal | Age | Isolation method | Region | Other cell type in the culture | Cellular visualization | Microscopy | Objective lens |
Resolution (x, y) z stack |
Interval time Total time |
References |
|---|---|---|---|---|---|---|---|---|---|---|
| SOD1-G93A mice (animal model of myotrophic lateral sclerosis) | 4 mo | Density gradient centrifugation | Whole brain | Motor neuron | Red (microglia) GFP (motor neuron) |
Confocal | – | – | – | Frakes et al. (89) |
| C57BL/6 mice | P3 | Mild trypsinization of mix glial coculture | Cortex | Bacteria | DIC | Epifluorescence | 10x or 20x | – | – | Hupp et al. (90) |
| mice or rats | P1-2 | Shaking of mix glial coculture | Cortex | Neuron | IsolectinB4-594 (microglia) YFP (neuron) |
– | – | – | – | Kraft et al. (91) |
| iPSCs | – | Differentiation | – | Synaptosome | DIC (microglia) pHrodo (synapptosome) |
Confocal | – | 0.61 × 0.61 μm – |
– | Sellgren et al. (92) |
iPSCs, induced Pluripotent Stem Cells; P, post-natal day; mo, month-old; –, not available.