Figure 3.

YAP participates in CAR activation-induced hepatomegaly and liver regeneration. (A) Total YAP, nuclear YAP and YAP downstream targets were all upregulated, while the cytoplasmic p-YAP was downregulated, which indicated the activation of YAP pathway. Proliferation-related proteins were also upregulated after treated by TCPOBOP. (B) In PHx model, total YAP, nuclear YAP and YAP downstream targets CTGF, ANKRD1 were upregulated, cytoplasmic p-YAP was downregulated. Meanwhile proliferation-related proteins such as CCND1 and CCNA1 were upregulated. (C) The intensity of nuclear YAP was much higher after treated by 10 μmol/L CITCO for 48 h in HepG2 cells, indicating that CAR activation promotes the nuclear translocation of YAP. (D) Co-immunoprecipitation results suggested the potential protein-protein interaction between CAR and YAP. Scale bar = 20 μm.