Skip to main content
. 2020 Nov 16;11(3):694–707. doi: 10.1016/j.apsb.2020.11.005

Figure 4.

Figure 4

Abrogation of USP7 sensitizes gastric cancer cells to T cell killing. (A) Binding of PD-1 with cell surface PD-L1 of MGC-803 cells when USP7 was abrogated genetically using sgRNA lentivirus or pharmacologically using Almac4 (5 μmol/L) for 4 days. (B) MGC-803 Con and USP7 KO cells were co-cultured with or without activated Jurkat cells for 4 days. Upper, the cells were stained with DAPI; down, the cell counts of MGC-803 Con and USP7 KO cells co-cultured with activated Jurkat cells. (C) MGC-803 Con (upper) and USP7 KO cells (down) were co-cultured with activated PBMC cells for 84 h. (D) The cell counts of (C). (E) Soluble IL-2 levels in MGC-803 Con and USP7 KO cells co-cultured with activated PBMC cells for 84 h. Data are shown as mean ± SD (n = 3); ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. P values were calculated using two-tailed t-test statistical analysis.