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. 2020 Nov 16;11(3):694–707. doi: 10.1016/j.apsb.2020.11.005

Figure 5.

Figure 5

Abrogation of USP7 suppresses GC cell proliferation and arrested cell cycle. (A) Knockout of USP7 in MGC-803 cells using two different sgRNA lentivirus. (B) Colony formation analysis when USP7 was knocked out in MGC-803 cells. (C) Expression of MDM2 and P53 when USP7 was knocked out in MGC-803 cells. (D) Cell cycle analysis when MGC-803 cells were treated with different concentrations of Almac4 for 48 h. (E) Colony formation when MGC-803 cells were treated with different concentrations of Almac4 for 10 days. (F) Expression of P53 and P21 in MGC-803 treated with different concentrations of Almac4 for 48 h. (G) Turnover of MDM2 in MGC-803 treated with Almac4 (5 μmol/L) for different times as indicated. Data are shown as mean ± SD (n = 3); ∗P < 0.05; ∗∗P < 0.01. P-values were calculated using two-tailed t-test statistical analysis.