Figure 6.

SHP2-WT thermal shift assay. (A) Protein/dye ratio optimization. Different protein/dye ratios were tested and the best one was chosen based on the principle of cost-saving and acceptable S/B ratio (S/B ≥ 4). (B) Thermal shift assay reliability verification by SHP-099. The unchanged curves of no protein control (NPC) and ligand only control (LOC) suggested there were no contamination in wells or protein melting reactions and ligand–dye interactions interference. Two concentrations (50 and 100 μmol/L) of SHP-099 were tested, whose ΔT_m values were 3.389 and 4.368 °C, respectively. Data are shown as mean ± SD; n ≥ 3.