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SHP2-PTP enzyme assay. (A) Linear enzyme concentration optimization of SHP2-PTP. 10 μmol/L DiFMUP was titrated with varying concentrations of SHP2-PTP ranging from 0.13 to 16.88 nmol/L. (B) Reliability verification of SHP2-PTP enzyme assay by control compound Na3VO4. Data are shown as mean ± SD; n ≥ 3.
