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CD spectra of the full-length and N-terminal deleted SARS 3CLpros.a, far-UV CD spectra of the full-length (▪) and N-terminal deleted (○) SARS 3CLpros at 25 °C. b, thermal induced unfolding monitored at 222 nm with a temperature range from 30 to 75 °C for the full-length (▪) and N-terminal deleted (○) SARS 3CLpros. Protein concentrations used in CD experiments were 10 μm, and all protein samples were prepared in 20 mm sodium phosphate, pH 7.5, 100 mm NaCl.
