TABLE 2.
The gradient elution of mobile phase of chromatography.
| Total time (min) | Flow rate (µL/min) | Ammonium acetate (%) | Methanol (%) |
|---|---|---|---|
| 0 | 600 | 95 | 5 |
| 0.5 | 600 | 95 | 5 |
| 1 | 600 | 20 | 80 |
| 1.5 | 600 | 20 | 80 |
| 1.51 | 600 | 95 | 5 |
| 3 | 600 | 95 | 5 |
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