TABLE 4.

Plackett–Burman experimental design and the observed responses.

Run	Independent variables																Dependent variables
	X1 (mg/ml)	X2 (mg/ml)	X3 (%)	X4	X5	X6 (°C)	X7 (°C)	X8 (rpm)	X9 (s)	X10 (°C)	X11 (rpm)	X12 (h)	X13	X14 (rpm)	X15 (h)	X16 (°C)	Y 1 (μm)	Y 2 (%)
1	700	250	3.0	1	200	10	30	800	60	25	100	2	1	500	0.5	30	128.0 ± 3.0	98.23 ± 0.31
2	700	250	0.1	1	50	10	30	3,000	10	45	100	5	2	500	0.5	10	94.4 ± 2.5	95.05 ± 0.43
3	700	75	0.1	1	200	10	10	800	60	45	500	5	2	100	0.5	30	122.0 ± 1.9	98.95 ± 0.45
4	700	75	0.1	5	50	25	30	800	10	25	100	5	2	500	3.0	30	165.0 ± 2.8	56.16 ± 0.38
5	150	75	3.0	5	50	10	30	3,000	60	25	500	2	2	500	0.5	30	26.2 ± 2.0	21.22 ± 0.27
6	150	250	0.1	5	50	10	10	800	60	45	500	2	2	500	3.0	10	55.5 ± 3.1	45.04 ± 0.27
7	150	250	3.0	5	200	10	10	800	10	25	100	5	2	100	3.0	30	17.6 ± 1.8	30.92 ± 0.30
8	700	250	0.1	5	200	10	30	3,000	10	25	500	2	1	100	3.0	10	45.3 ± 2.2	87.24 ± 0.25
9	150	75	0.1	5	200	25	10	3,000	10	45	100	2	1	500	0.5	30	48.5 ± 1.7	59.27 ± 0.36
10	700	250	3.0	1	50	25	10	800	10	45	500	2	1	500	3.0	30	126.0 ± 2.9	97.09 ± 0.28
11	700	75	3.0	1	50	25	10	3,000	60	25	100	2	2	100	3.0	10	33.5 ± 2.1	68.15 ± 0.31
12	150	250	0.1	1	200	25	30	3,000	60	45	500	2	2	100	3.0	30	26.7 ± 3.2	47.91 ± 0.28
13	700	75	3.0	5	200	25	30	800	10	45	100	2	2	100	0.5	10	56.3 ± 2.2	66.81 ± 0.27
14	150	250	3.0	5	50	25	30	800	60	45	500	5	1	100	0.5	10	15.9 ± 1.8	28.66 ± 0.30
15	150	75	0.1	1	200	25	30	800	60	25	100	5	1	500	3.0	10	101.0 ± 3.4	63.36 ± 0.24
16	150	75	0.1	1	50	10	10	800	10	25	100	2	1	100	0.5	10	112.0 ± 2.9	70.67 ± 0.29
17	150	250	0.1	5	200	25	10	3,000	10	25	500	5	2	500	0.5	10	15.3 ± 2.0	62.33 ± 0.32
18	150	75	0.1	1	50	10	30	3,000	10	45	500	5	1	100	3.0	30	18.1 ± 2.9	46.15 ± 0.28
19	700	250	3.0	5	50	10	10	3,000	60	25	500	5	1	100	0.5	30	39.4 ± 2.2	67.51 ± 0.25
20	700	75	3.0	1	200	25	10	3,000	60	45	100	5	1	500	3.0	10	43.2 ± 2.1	69.59 ± 0.19