FIGURE 4.

HIF‐1α activates CFL1 transcription in HCC cells. (A) CFL1 protein expression was upregulated in HCCLM3 and Hep3B cells under hypoxic conditions, which could be abolished by HIF‐1α knockdown. (B) HIF‐1α silencing reversed hypoxia‐induced CFL1 mRNA expression in HCC cells. (C) CFL1 protein expression was upregulated in HCCLM3 and Hep3B cells under hypoxic conditions, which could not be abolished by HIF‐2α knockdown. (D) HIF‐2α silencing could not reverse hypoxia‐induced CFL1 mRNA expression in HCC cells. (E,F) HIF‐1α inhibitors (digoxin and acriflavine) consistently reduced the CFL1 level in HCC cells under hypoxic conditions. (G) ChIP assay revealed that HIF‐1α and HIF‐1β directly bond to the CFL1 promoter in HCC cells. (H) HRE luciferase activities were promoted in 293T cells transfected with HRE‐luciferase‐plasmid in hypoxic condition. *p < 0.05