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. 2021 Mar 8;12:625562. doi: 10.3389/fmicb.2021.625562

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Copyright © 2021 Jang, Son, Kim, Jung, Choi, Kim, Lee, Shin and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial identification using 16S metagenomics approach. Eighty-eight bacterial strains were identified from dog feces. Molecular phylogenetic analysis showing their relationship, including CACC517, CACC537, CACC558, and CACC566, is displayed using the maximum-likelihood method (Ding and Shah, 2007; Guo et al., 2016). Bootstrap values of 500 replicates are shown at the tree nodes, as generated using MEGA 7. The scale bar corresponds to 0.02 units of the number of base substitutions per site (A). Venn diagram plot represents three parts (core, accessory, and strain-specific) of the pan-genome. The center (422), overlapped (2∼4 times) and outer (non-overlapped) indicate the number of core, accessory and strain-specific genes respectively (B).