Table 3.
Assay medium glycolysis stress test
| Reagent | Default assay medium compositiona | Optimized assay medium composition for intestine-derived organoidsb | Volumes to prepare 50 mL optimized assay medium |
|---|---|---|---|
| Glutamine (200 mM) | 2 mM | 2 mM | 0.5 mL |
| NaOH (1 M) | Adjust to pH 7.4 | Adjust to pH 7.4 | 25 μL |
| Base medium | n/a | n/a | 49.5 mL |
| Total | n/a | n/a | 50 mL |
a
Default assay medium composition suggested by Agilent.
b
Assay medium composition optimized for mouse small intestine- and human colon- or colorectal tumor-derived organoids.