Table 4.

Mitochondrial stress test injections optimized for mouse small intestine-, human colon- and colorectal tumor-derived organoids

Injection port	Drug	Final concentration in Seahorse plate	Injection concentration	To add from 2.5 mM stock (μL)	XF assay medium (μL)
Port A	Oligomycin	5 μM	40 μM	32	1,968
Port B	FCCP	2 μM	18 μM	14.4	1,985.6
Port C	Rotenone and Antimycin A	2 μM + 2 μM	20 μM	16 (each)	1.68
Port D