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. 2021 Mar 5;8:606197. doi: 10.3389/fvets.2021.606197

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Copyright © 2021 Massimini, Dalle Vedove, Bachetti, Di Pierro, Ribecco, D'Addario and Pucci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The relative expression of canine atopic dermatitis (AD) marker genes after the nutraceutical mixture treatment in CPEK cells. Inflamed cells were treated (Mix yes) or not (Mix no) with the nutraceutical mixture (Mix 2) for 4 and 8 h. The bar graphs show the mean + SEM of three independent experiments performed in triplicate. The gene expression of ccl2 (A), ccl17 (B), tslp (C), and il4r (D) was calculated using the delta–delta Ct (ΔΔCt) method. The statistically significant differences between experimental conditions are reported as follows: *p < 0.05, **p < 0.01, ***p < 0.001 vs. no mix 4 h; §§ p < 0.01, vs. yes mix 4 h; °°°p < 0.001 vs. no mix 8 h.