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. 2021 Mar 5;8:606197. doi: 10.3389/fvets.2021.606197

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Copyright © 2021 Massimini, Dalle Vedove, Bachetti, Di Pierro, Ribecco, D'Addario and Pucci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of the nutraceutical mixture on modulated gene CpG island methylation in DH82 cells. Inflamed cells were treated (Mix yes) or not (Mix no) with nutraceutical mixture (Mix 2) for 4 and 8 h. The bar graphs represent the mean + SEM of three independent experiments performed in triplicate. The figure shows the methylation percentage with respect to ccl2 (A–C), ccl17 (D), and il31ra (E) expressed as methylated cytosine/methylated cytosine + unmethylated cytosine. The statistically significant differences between experimental conditions are reported as follows: *p < 0.05; ^§p < 0.05, ^§§§p < 0.001 vs. yes mix 4 h; °p < 0.05, °°p < 0.01 vs. no mix 8 h.