Table 2.

Primer sequences used to analyze DNA methylation levels of canine genes.

Gene	Forward (5′ → 3′)
ccl2	GGGAAGTTTTGTGTATTTTATAGGTATTG
ccl17	AGGGAATTAGAGGGAAGGTAGGA
il31ra	GAGGGTTTAAGAATAGATTTTATTGTAGGT
tslp	TTTTAGTAAGTGTTATAGGGGTTAAGAGAT
Gene	Reverse biotinylated (5′ → 3′)
ccl2	AACAAAAAAAAAAACAAACTCCATAC
ccl17	TAAAAACCCCCAACAACCTCC
Il31ra	AAACCCCCTACCACCTTATCTT
tslp	ACTCCCTCCCCTAAACTACAAC
Gene	Sequencing (5′ → 3′)
ccl2	AATGATTTTATAAGAAAGAAGATT
ccl17	GGTTGGAAGGGAAAG
il31ra	AATAGATTTTATTGTAGGTATT
tslp	GGGGTTAAGAGATTTTTG