TABLE 1.
Control | DHEA (3 weeks) | DHEA (7 weeks) | DHEA + BHG (0.75 g/kg) | DHEA + BHG (1.49 g/kg) | DHEA + BHG (2.99 g/kg) | DHEA + KW (0.46 g/kg) | DHEA + KW (0.91 g/kg) | Total | |
Observation of estrous cycle15 | – | 15 | 15 | 15 | 15 | 15 | 15 | 15 | 120 |
HE staining(3) | 3 | (3) | (3) | (3) | (3) | (3) | (3) | (3) | 3, (24) |
Testosterone and insulin in serum(6) | 6 | (6) | (6) | (6) | (6) | (6) | (6) | (6) | 6, (48) |
TUNEL staining(3) | (3) | (3) | (3) | (3) | (3) | (3) | (3) | (3) | (27) |
MitoSOX(3) | (3) | (3) | (3) | (3) | (3) | (3) | (3) | (3) | (27) |
Mitochondrial respiratory chain complexes activities8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 72 |
Western blot(8) | (8) | (8) | (8) | (8) | (8) | (8) | (8) | (8) | (72) |
Pregnancy outcome20 | – | 18 | 10 | 10 | 9 | 8 | 10 | 10 | 95 |
Total43 | 17 | 41 | 33 | 33 | 32 | 31 | 33 | 33 | 296 |
Numbers in brackets represent the number of the animals used in the respective experiment, and these animals were among those denoted above by the numbers free of brackets.