Skip to main content
. 2021 Mar 2;12:637164. doi: 10.3389/fimmu.2021.637164

Figure 5.

Figure 5

Classification of the SA/Malawi cohorts using the DRS based on the FS-PLS signature RT-dPCR results. (A) Box plots of disease risk score (DRS) (TB vs. OD DRS p = 1.34 × 10−7) and (B) Receiver operating characteristic (ROC) curve based on the TB/OD FS-PLS signature applied to the combined TB and OD cohorts (n TB = 20, n OD =20). Gray shaded areas represent the 95% CIs of the ROC curve sensitivities, plotted at 0.5% specificity intervals. The ROC curve has been benchmarked against target criteria for a tuberculosis triage test: Minimum criteria (90% sensitivity, 70% specificity) are indicated by the dashed green boxes and optimum criteria (95% sensitivity, 80% specificity) are indicated by the green solid boxes; and the minimum criteria for a confirmatory test (65% sensitivity, 98% specificity) which are indicated by the orange box. (C) Box plots of DRS (TB vs. OD DRS HIV uninfected p = 1.05 × 10−3, HIV infected p = 4.871 × 10−4), and (D) ROC curve for TB/OD signature, according to HIV infection status (red line is HIV infected; blue line is HIV uninfected) (n TBHIV− = 10, n TBHIV+ = 10, n ODHIV− = 10, n ODHIV+ = 10). Blue and orange shaded areas represent the 95% CIs of the ROC curve sensitivities, plotted at 0.5% specificity intervals. (E) Box plots of DRS (TB vs. LTBI DRS p = 2.83 × 10−9) and (F) ROC curve based on the TB/LTBI FS-PLS signature applied to the combined TB and LTBI cohorts (n TB = 20, n LTBI = 20). Gray shaded areas represent the 95% CIs of the ROC curve sensitivities, plotted at 0.5% specificity intervals. The ROC curve has been benchmarked against target criteria for a tuberculosis triage test: Minimum criteria (90% sensitivity, 70% specificity) are indicated by the dashed green boxes and optimum criteria (95% sensitivity, 80% specificity) are indicated by the green solid boxes. (G) Box plots of DRS (TB vs. LTBI DRS HIV uninfected p = 1.083 × 10−5, HIV infected p-value 3.248 × 10−4, and (H) ROC curve for TB/LTBI signature, according to HIV infection status (red line is HIV infected; blue line is HIV uninfected) (n TBHIV− = 10, n TBHIV+ = 10, n LTBIHIV−= 10, n LTBIHIV+ =10). 95% confidence intervals are shown in brackets. The orange shaded area represents the 95% CIs of the ROC curve sensitivities, plotted at 0.5% specificity intervals. The blue shaded area for the perfect classifier represents the 95% CI for sensitivity. All p-values reported are Mann–Whitney p-values. AUC, area under the curve; OD, other diseases; LTBI, latent TB infection.