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. 2021 Mar 3;11:657868. doi: 10.3389/fonc.2021.657868

Figure 6.

Figure 6

Production of interferon-γ (IFN-γ) and cell proliferation non-transduced (NT) T cells, A20-2G CAR T cells, and A20-4G CAR T cells after co-culture with integrin αvβ6-expressing target cells. (A) IFN-γ production was examined by intracellular cytokine staining and flow cytometry analysis in NT T cells, A20-2G CAR T cells, and A20-4G CAR T cells after co-culturing with integrin αvβ6-negative A375.puro or integrin αvβ6-positive A375.β6 cells. (B) IFN-γ expression from three individual healthy donors. Data are presented as mean ± standard error of the mean (SEM). (C) Proliferation of NT cells, A20-2G CAR T cells, and A20-4G CAR T cells was examined after co-culturing for 3 days with integrin αvβ6-negative A375.puro or integrin αvβ6-positive A375.β6 cells at an effector to target (E:T) ratio of 5:1 without addition of exogenous cytokine. (D) Summary data of T cell proliferation derived from 3 individual healthy donors. (*p < 0.05, **p < 0.01, ***p < 0.001).