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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Neurobiol Dis. 2020 May 4;141:104934. doi: 10.1016/j.nbd.2020.104934

Fig. 2.

Fig. 2.

Consumption of a Western diet impairs recovery of sensorimotor and bladder function after SCI and is associated with reductions in spared spinal cord tissue volume. (A–C) Recovery of sensorimotor function assessed by the Basso Mouse Scale (BMS) score (A) and BMS subscore (B), and the Inclined Plane test (C) were reduced in mice consuming HFHS compared to those consuming a RD. (D) Signs of spontaneous bladder release also showed delayed recovery in mice consuming HFHS compared to RD at wk. 1, 3 and 4 after SCI (RD, n = 9 and HFHS, n = 10, Two-way ANOVA, *P < .05, **P < .01, ***P < .001). (E–G) Application of the Cavalieri estimator probe to Eriochrome and neurofilament dual stained spinal cord sections taken from 2 mm blocks encompassing the injury epicenter (0), and 4 mm above and below, showed no differences in the volume of white matter damage or in the volume of spared white matter at 30 dpi across groups (RD, uninjured n = 5, 14 dpi n = 4, 30 dpi n = 5 and HFHS, uninjured n = 5, 14 dpi n = 5, 30 dpi n = 5, *P < .05, **P ≤ .01, ***P < .001, Two-way ANOVA). RD = regular diet, HFHS = high-fat high-sucrose.