Table 3.
Primers used for quantitative real-time PCR.
| Gene | Accession number | Probe and primer information |
|---|---|---|
| ALDH1L1 | NM_027406.1 | Applied Biosystems, Assay ID: Mm03048957_m1 |
| GAP43 | NM_008083.2 | Applied Biosystems, Assay ID: Mm00500404_m1 |
| GFAP | NM_010277.2 | GCAGATGAAGCCACCCTGG/ GAGGTCTGGCTTGGCCAC |
| Iba-1 | NM_019467.2 | Applied Biosystems, Assay ID: Mm00479862_g1 |
| IGF-1 |
NM_001111274.1, NM_001111275.1, NM_001111276.1, NM_010512.4, NM_184052.3 |
Applied Biosystems, Assay ID: Mm00439560_m1 |
| IGF-1 receptor | NM_010513 | Applied Biosystems, Assay ID: Mm00802831_m1 |
| IGF-2 | NM_001122736.1, NM_001122737.1, NM_010514.3 | Applied Biosystems, Assay ID: Mm00439564_m1 |
| IL-6 | NM_031168.1 | Applied Biosystems, Assay ID: Mm00446190_m1 |
| Insulin | NM_008386.3 | Applied Biosystems, Assay ID: Mm01950294_s1 |
| Insulin receptor | NM_010568.2 | Applied Biosystems, Assay ID: Mm01211875_m1 |
| MBP | NM_001025251 | CCAGTAGTCCATTTCTTCAAGAACAT/GCCGATTTATAGTCGGAAGCTC |
| NFH | NM_010904.3 | CATTGAGATTGCCGCTTACAG/ TTATGTGCGTGGATATGGAGG |
| Olig2 | NM_016967 | IDT, Assay ID: Mm.PT.58.42319010 |
| PGC1 alpha | NM_008904.2 | Applied Biosystems, Assay ID: Mm01208835_m1 |
| PLP | NM_011123.2 | TCTTTGGCGACTACAAGACCAC/CACAAACTTGTCGGGATGTCCTA |
| Rn 18S | NR_003278.3 | Applied Biosystems, Assay ID: Mm03928990_g1 |
| SIRT1 | NM_001159589.1, NM_019812.2 | Applied Biosystems, Assay ID: Mm00490758_m1 |
| Synapsin-1 | NM_001110780.1 | Applied Biosystems, Assay ID: Mm00449772_m1 |