Figure 1.

Endothelial cell-restricted endothelin-1 overexpression worsened atherosclerosis in type 1 diabetes through NOX1. Atherosclerotic lesion areas were determined by oil red O staining in aortic sinus (A, B) and arch (C, D) of Apoe^−/−, eET-1/Apoe^−/−, eET-1/Apoe^−/−/Nox1^−/y, and eET-1/Apoe^−/−/Nox4^−/− mice 14 weeks after intraperitoneal injections with either vehicle (Veh) or streptozotocin (STZ). Representative images of oil red O-stained and Mayer’s haematoxylin-counterstained aortic sinus (A) and arch (C) cryosections are shown. Lesion sizes were calculated as mean μm² of four sections obtained at 90-μm intervals of aortic sinus or arch. Data are presented as means ± SEM, n = 5–9. Data were analysed using one-way ANOVA followed by a Student–Newman–Keuls post hoc test. *P < 0.05 and **P < 0.001 vs. non-diabetic Apoe^−/−, ^†P < 0.01 and ^††P < 0.001 vs. diabetic Apoe^−/−, ^‡P < 0.01 and ^‡‡P < 0.001 vs. non-diabetic eET-1/Apoe^−/−, ^§P < 0.05 and ^§§P < 0.001 vs. diabetic eET-1/Apoe^−/−.