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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Mod Pathol. 2020 Oct 1;34(3):562–571. doi: 10.1038/s41379-020-00686-6

Fig. 1. Digital selection of regions of interest (ROIs) in QuPath relies on the size of the tumor.

Fig. 1

(a) Melanoma tumors that are ≤1 mm2 in histologic area can be assessed using a single fully encompassing ROI. (b) Larger tumors (>1 mm2) require the selection and averaging of multiple, smaller ROIs for cell segmentation and % TIL quantification due to computational limitations.