Fig. 5.

Treatment-induced changes in PCO areas. Quantitative image analysis tracked changes in the area of each PCO over time. (a) Representative segmented image time series for patient 1 (P1) and patient 2 (P2). The color-coded value of each segmented organoid corresponds to value at each time point divided by its pretreatment value. Arrows indicate specific organoids that are representative of changes observed for that condition. (b) Heatmaps show the pairwise percent differences between pretreatment-normalized area between all treatment groups for P1. Top: pairwise percent differences between each treatment and control for P1. Bottom: pairwise percent differences between drug treatments (excluding control) for P1. (C) Heatmaps show the pairwise percent differences between pretreatment-normalized area between all treatment groups for P2. Top: pairwise percent differences between each treatment and control for P2. Bottom: pairwise percent differences between drug treatments for P2. All pairwise differences were calculated from the linear mixed-effect models via least-squares means. n.s., not significant ($p>0.05$, black boxes). Table 1 shows the number of PCO in each treatment group for each patient. Scale bar: $500\mu \mathrm{m}$.