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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Cell Signal. 2018 Mar 24;47:131–141. doi: 10.1016/j.cellsig.2018.03.008

Fig. 1. 2-D Western blot analysis with anti-phosphotyrosine antibody of lysates from IL-7-stimulated D1 cells.

Fig. 1.

Soluble protein fractions from lysates of unstimulated or IL-7 stimulated D1 cells were analysed by 2D Western blot analysis and Coomassie blue gel staining as described in Materials and Methods. A, Unstimulated cells, Coomassie blue staining; B, Unstimulated cells, immunoblot with anti-phosphotyrosine MoAb; C, IL-7-stimulated cells, Coomassie blue staining; D, IL-7-stimulated cells, immunoblot with anti-phosphotyrosine MoAb. Spots are numbered as reported in Table 1, showing protein identification. Representative of 3 experiments.