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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Cell Signal. 2018 Mar 24;47:131–141. doi: 10.1016/j.cellsig.2018.03.008

Fig. 3. Tyrosine phosphorylation of CrkL in IL-7-stimulated murine and human T cells and in murine pro-B cells, and association of CrkL with Stat-5 in IL-7 stimulated human T cells.

Fig. 3.

Western blot analysis with anti-phospho-CrkL, anti-phospho-Jak1, and anti-CrkL polyclonal antibodies. A, Murine T cells purified from lymph nodes as described in Materials and Methods were incubated with or without IL-7 for the indicated times. Representative of 3 experiments; B, Human T lymphocytes purified as described in Materials and Methods were incubated with or without IL-7 for the indicated times. Representative of 3 experiments; C, Pro-B cells purified from spleens of Rag1-deficient mice as described in Materials and Methods were incubated with or without IL-7 for 15 min. Representative of 3 experiments; D, Coimmunprecipitation on cell lysates from unstimulated and IL-7 stimulated human T cells was performed with anti-CrkL antibody as described in Materials and Methods. Blots were probed with anti-Stat5 and anti-CrkL antibodies. Representative of 3 experiments.