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. Author manuscript; available in PMC: 2021 Mar 22.
Published in final edited form as: Cell Signal. 2018 Mar 24;47:131–141. doi: 10.1016/j.cellsig.2018.03.008

Fig. 5. Expression of Crk I and II proteins by CrkL conditionally-deficient pro-B cells and apoptosis of conditionally-deficient Crk/CrkL pro-B cells.

Fig. 5.

A: Western blot analysis with anti-CrkL and anti-Crk MoAb performed in duplicate. Purified pro-B cell bearing loxP-flanked CrkL alleles transduced with pMSCV-Cre recombinase-IRES-GFP in the presence of IL-7 for 67 h were sorted in GFP+ and GFP− cells. Representative of 3 experiments;B: Pro-B cells bearing loxP-flanked Crk/CrkL alleles were transduced with pMSCV-Cre recombinase-IRES-GFP or with the pMSCV-IRES-GFP empty vector in the presence of IL-7 and analysed after 5 days. The percentage of apoptotic cells was 43.19 and 17.54%, respectively. Representative of two experiments.