Figure 7.

Gal3 is associated with the alterations of the mRNA levels of pro-angiogenic and apoptotic genes in neuronal cells under in vitro ischemic conditions.

(a) Determination of angiogenesis and apoptosis-related gene expression changes by quantitative real-time polymerase chain reaction using endothelial biology pathway specific PCR array. (b) Validation of changes in mRNA levels of selected genes by semi quantitative PCR analysis. Pretreatment with Gal3 increases mRNA levels of pro-angiogenic factors occludin and Platelet endothelial cell adhesion molecule (PECAM1)/CD31, and pro-survival molecule BCL2. It decreases mRNA levels of pro-apoptotic Fas ligand in neuronal cells exposed to oxygen glucose deprivation (in vitro ischemia). Levels of beta-actin mRNA were used as internal control. (c) Quantitative analysis showing reduced/increased expression of occludin, PECAM1/CD31 (Ca), BCL2, and Fas ligand (Cb). Bars represent the gene expression normalized to beta-actin. **P<0.01 vs. control (N = 3).