Fig. 2.

Extramammary Paget disease in the scrotal skin (A−G) and bladder trigone (H–N) stains with CK7 (B, I) with minimal tumor expression of major histocompatibility complex class I (MHCI) (C, J), predominantly cytoplasmic tumor beta-2 microglobulin (B2M) (D, K), no tumor expression of major histocompatibility complex class II (MHCII) (E, L) and no tumor expression of programmed death-ligand 2 (PD-L2) (F, M) or programmed death-ligand 1 (PD-L1) (G, N) with minimal expression in the tumor microenvironment. Control immunohistochemistry (IHC) is shown using breast carcinoma for CK7 (O), lymph node for MHCI (P), B2M (Q), MHCII (R) and PD-L2 (S) and placenta for PD-L1 (T). All images were taken at ×20. A 50 μm scale bar is shown in T.