Figure 3.

IL‐38 expression in PBMC and keratinocytes. (A) IL1F10 and GAPDH mRNA expression was detected by RT‐qPCR in keratinocyte cultures that were either undifferentiated, overconfluent, or terminally differentiated, as well as resting PBMC isolated from venous blood, isolated CD19⁺ B cells from these PBMC, and the CD19⁺ depleted flow‐through. Expression of IL1F10 was normalized to GAPDH using the 2^−ΔΔCt method. (B) IL‐38 protein expression was measured in cell lysates by ELISA and expressed as pg IL‐38 per mg total protein. (C) IL‐38 protein in the supernatant of CD19⁺ enriched or depleted PBMC after 24 h and 48 h stimulation with rituximab and measured by ELISA. Data are expressed as mean ± SEM from three independent experiments with a total of three donors of keratinocytes and PBMC that were analyzed in parallel by RT‐qPCR and ELISA. N.d.: not detected. Unpaired T test, *p < 0.05, **p < 0.01.