FIGURE 1.

Microscopic analysis of NP cells during the first and second phase of Expansion. A‐L, Microscopic analysis of human NP cells seeded within different conditions. A, E, and I, Human NP cells seeded in two‐dimensional (2D) with a culture medium supplemented with ascorbic acid. B, F, J, Human NP cells seeded in three‐dimensional (3D) (alginate beads) with a culture medium supplemented in ascorbic acid. C, G, K, Human NP cells seeded 2D and then in 2D on fibronectin‐coated flasks with fibroblast growth factor (FGF‐2) supplemented culture medium. D, H, L, Human NP cells seeded 3D and then in 2D on fibronectin‐coated flasks with FGF‐2 supplemented culture medium. Scale bar: For pictures, A, until, L, the scale bar represents 150 μm