Table 1.
The determined T m and T agg values of WT LEH and LEH variants based on collected label‐free fluorescence, DSF and SLS.
|
Sample |
Mutations |
Predicted T 50 [°C] |
Expl. T m [°C] |
Expl. T agg [°C] |
|---|---|---|---|---|
|
WT LEH |
|
41 |
46.38±0.01 |
42.6±0.06 |
|
LEH‐1 |
S15P/A19K/T85V/G89C/S91C/L114V/E124D |
69.14 |
65.95±0.36 |
53.4±0.02 |
|
LEH‐2 |
S15P/A19K/T85V/G89C/S91C/L114V/I116V/E124D |
69.04 |
60.09±0.02 |
56.6±0.19 |
|
LEH‐3 |
I5C/S15P/A19K/L74F/T85V/G89C/S91C/L114V/I116V/E124D |
66.21 |
73.62±0.33 |
63.3±0.00 |
|
LEH‐4 |
S15P/A19K/M78F/I80V/T85V/G89C/S91C/N92K/L114V/I116V/F139V/L147F |
66.13 |
61.31±0.33 |
49.3±0.10 |
|
LEH‐5 |
S15P/A19K/M78F/I80V/T85V/G89C/S91C/Y96F/L114V/I116V/E124D/F139V/L147F |
66.07 |
71.30±0.33 |
62.6±0.07 |
|
LEH‐F1b |
I5C/S15P/A19K/T76K/E84C/T85V/G89C/S91C/N92K/Y96F/E124D |
63 |
63.12±0.46 |
55.6±0.21 |