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DNA cleavage activity of bis‐3‐chloropiperidines a) 5 versus 6 and b) 7 versus 8. The supercoiled pBR322 plasmid was incubated with increasing concentrations of test compounds at 37 °C for 3 h in BPE buffer. Lower band: supercoiled plasmid form, upper bands: open circular plasmid species. C: supercoiled pBR322 plasmid control.
