Figure 4.

DNA cleavage activity of bis‐3‐chloropiperidines 8–9 after overnight preincubation of compounds at 37 °C in BPE buffer (pH 7.4). Preincubated compounds were added at increasing concentrations to the pBR322 plasmid, and the mixture was incubated at 37 °C for 3 h. C: supercoiled pBR322 plasmid control.