TABLE 1.

Media conditions with number of replicates, average final pH, average amplicon strain variants (ASVs), and average species numbers

Condition			Replicates	Average Final pH ± SD	Average ASVs ± SD	Average species ± SD
% FBS	% sucrose	g/L mucin
0	0	2.5	1	7.2	64	50
0	0.1	2.5	5	6.88 ± 0.38	51.8 ± 12.24	43.8 ± 8.04
0	0.5	2.5	3	4.53 ± 0.15	46.7 ± 10.26	41.3 ± 8.39
0	0.8	2.5	2	4.65 ± 0.07	51 ± 12.73	44.5 ± 9.19
10	0	2.5	3	7.23 ± 0.12	57.33 ± 2.08	44 ± 2.65
10	0.1	2.5	3	6.3 ± 0.70	59.3 ± 20.98	49.3 ± 16.65
10	0.5	2.5	2	4.5 ± 0.14	38.5 ± 4.95	35.5 ± 6.36
10	0.8	2.5	2	4.7 ± 0	41 ± 8.49	38 ± 8.49
20	0	2.5	3	6.73 ± 0.12	63 ± 9.64	46.7 ± 5.51
30	0.1	2.5	1	6.1	40	35
50	0.1	2.5	1	5.6	28	24
10	0	0	3	7.47 ± 0.06	57.33 ± 2.89	45.67 ± 2.31
0	0.1	0.1	1	7.1	55	44
30	0.1	0.1	3	5.23 ± 0.06	24 ± 4.36	22.33 ± 3.79
0	0.1	8.0	1	6.4	49	43

subSHI‐v1 medium is marked in bold.

Abbreviation: SD, standard deviation.