Table 2. FGF14 phosphomotifs correspond to hit targets identified by HTS.

Subsequent investigation using the HPRD PhosphoMotif Finder, NetPhos 3.1, and NetPhorest 2.1 revealed potential JAK2 and Src phosphorylation sites at Y158 and Y162, respectively, as well as other sites for kinases identified in the HTS.

FGF14 Position	FGF14 Sequence	Kinase or Phosphatase	FGF14 Position	FGF14 Sequence	Tyrosine Binding Motifs
81 – 86	YCRQGY	ALK	81 – 84	pYCRQ	STAT3
145 – 147	TPE	p38 MAPK	86 – 89	pYYLQ	STAT3
158 – 161	YYVI	JAK2	129 – 132	pYIAM	PI3K p85
162–163	YS	Src	156 – 161	SVFENpYYVIYS	SHC, SH2
162 – 167	YSSMLY	ALK, INSR	154 – 165	VFENYpYVIYSS	SH2, PTP
193 – 195	KKT	PKC	162 – 165	pYSSM	PI3K p85
211 – 212	YR	Src	167 – 170	pYRQQ	STAT3
226 – 230	SKSTS	GSK3	206 – 216	LEVAMpYREPSL	SH2, PTP