Table 4. High affinity FGF14:FGF14 dimerization is abolished by phosphorylation by JAK2, and by Src to a lesser extent.

Kinetic constants calculated from data represented in Figure 3B. The K_D represents the average between the kinetic K_D, calculated using the simplest Langmuir 1:1 interaction model (K_D=k_off/k_on), and the steady-state saturation (affinity) K_D. Data are mean ± SD.

	KD (nM)	kon (M−1 s−1)	koff (s−1)
FGF14:FGF14	440 ± 57	1.36×104 ± 4.9×102	9.04×10−4 ± 9.2×10−5
FGF14:FGF14+JAK2	2701 ± 97	4.02×103 ± 5.6×102	4.52×10−3 ± 3.1×10−4
FGF14:FGF14+Src	1312 ± 84	9.06×103 ± 3.5×102	1.33×10−3 ± 4.9×10−4