Fig. 9.

Lotus japonicus NF‐YA1 and NF‐YA4 work partially redundantly to regulate expression of STY genes. The steady‐state levels of L. japonicus STY mRNAs in control, uninoculated (UN) roots of L. japonicus wild‐type (black), nf‐ya1‐2 (dark grey) and nf‐ya1‐2 nf‐ya4 mutants (light grey) and in corresponding root samples collected at 4 and 7 d after inoculation (dai) with Mesorhizobium loti. The mean ± SE is given for four to five biological replicates. Statistical groupings across different time points, reflected by the same letters, have been determined separately for each genotype using one‐way ANOVA with Tukey’s honestly significant difference post hoc test (P < 0.05). Dunnett’s test was used to carry out pairwise comparisons between wild‐type, nf‐ya1‐2 and nf‐ya1‐2 nf‐ya4 mutants at each time point (*, P < 0.05; **, P < 0.01; ***, P < 0.001).