Table 1.
Method of Conventional Pap stain and REAP staining[7]
| Conventional Pap Stain | REAP | ||
|---|---|---|---|
| 95% alcohol (for fixation) | 95% alcohol (for fixation) | ||
| 95% alcohol | 10 dips | 1% acetic acid | 10 dips |
| 80% alcohol | 10 dips | – | |
| 70% alcohol | 10 dips | - | |
| 50% alcohol | 10 dips | – | |
| Tap water | 10 dips | - | |
| Harris haematoxylin | 2 minutes | Harris Haematoxylin, preheated60°C | 10 dips |
| Scott’s Tap water | 3-5 min | Tap water | 10 dips |
| 50% alcohol | 10 dips | – | |
| 70% alcohol | 10 dips | – | |
| 80% alcohol | 10 dips | 1% acetic acid | 10 dips |
| 95% alcohol | 10 dips | – | |
| OG 6 | 1 min | OG 6 | 10 dips |
| 95% alcohol | 10 dips | – | |
| 95% alcohol | 10 dips | 1% acetic acid | 10 dips |
| EA | 10 min | EA | 10 dips |
| 95% alcohol | 40 dips | 1% acetic acid | 10 dips |
| 95% alcohol | 40 dips | – | |
| 100% alcohol | 10 dips | Methanol | 10 dips |
| Xylene | 10 dips | Xylene | 10 dips |
| Xylene | 10 dips | – | |
| Xylene | 10 dips | – | |
| DPX mount | Coverslip | DPX mount | Coverslip |